BSC1005 Study Guide for Laboratory

Laboratory Study Guide,

Reproduction: Molecular to Cellular

Illustration from University of Arizona, The Biology Project.

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Read:

Reproduction: Molecular to Cellular, pp 1-6.


Terms to Know in This Chapter:

reproduction [re pro DUCT shun]

mitosis [my TOE siss]

chromosome (sing.) [CROW moe zome]

nucleus (sing.) [NEW klee us]

nuclei (pl.) [NEW klee eye]

gene (sing.) [JEAN]

histone (sing.) [HIS tone]

deoxyribonucleic acid [dee OX ee RYE bow new CLAY ik]

replication [rep leh KAY shun]

onion [ON yun]

fixative [FIX ah tive]

preservative [pre SERVE ah tive]

acetoorcein [ah SEAT oh OR seen]

spindle [SPIN dul]

interphase [IN tur faze]

prophase [PRO faze]

metaphase [MET ah faze]

anaphase [AN ah faze]

telophase [TEL oh faze]

daughter cells

nucleolus (sing.) [new KLEE oh lus]

nucleoli (pl.) [new KLEE oh lie]

centromere (sing.) [SIN trow meer]

centriole (sing.) [SIN tree ole]

ribonucleic acid [RYE bow new CLAY ik]

Gap 1

Gap 2

synthesis [SIN theh siss]

chromatin [CROW mah tin]

chromatid (sing.) [crow mah TID]

haploid [HAP lloyd]

diploid [DIP lloyd]

cleavage furrow [CLEE vage] [FUR row]

cell plate

adenine [ADD eh neen]

thymine [THIGH meen]

cytosine [SIGH tow seen]

guanine [GWAH neen]

nucleotide (sing.) [NEW klee oh tide]

double helix


Genera You Need to Know

Allium [AL ee um]

People You Need to Know

Virchow [VEER chow]

Flemming [FLEM ing]

Watson

Crick

What You Need to Know:


You should be able to:

  1. some of the contributions of scientists to mitosis and cell reproduction,
  2. describe the structure of DNA,
  3. explain the cell cycle,
  4. demonstrate the ability to prepare a slide of mitosis, fix, preserve and stain it, and identify the various stages of the cell cycle with your slide,
  5. explain the events of interphase, prophase, metaphase, anaphase, and telophase, and
  6. distinguish between haploid and diploid.

Exercises: Fill in the Blank.


  1. The two people who first proposed the accurate structure of DNA were and
  2. 6N HCl with 95% EtOH serves as a in the preparation of the Allium root tip.
  3. HAc with 70%EtOH serves as a in the preparation of the mitosis slide.
  4. The stain used to highlight the nuclei of cells is .
  5. is the stage of the cell cycle where the centromeres divide and the chromosomes push apart.
  6. The stage of the cell cycle where the spindle forms is .
  7. The stage of the cell cycle where the DNA replicates is .
  8. Cell plate formation takes place in this part of the cell cycle. .
  9. Chromosomes become visibly thickened during this stage of the cell cycle.
  10. In DNA, the base adenine always pairs with the base .
  11. If the original cell is diploid, then each daughter cell that results from mitosis is (haploid, diploid).
  12. The point where two chromatids are joined is called the .


Exercises: Multiple Choice. Select the Best Answer.


  1. Who recognized that reproduction is a fundamental process of cells?

    (1) Virchow

    (2) Flemming

    (3) Watson

    (4) Crick

    (5) Watson and Crick

  2. The nucleoli disappear during what stage of the cell cycle?

    (1) interphase

    (2) prophase

    (3) metaphase

    (4) anaphase

    (5) telophase

  3. During what stage of the cell cycle do the centrioles divide?

    (1) interphase

    (2) prophase

    (3) metaphase

    (4) anaphase

    (5) telophase

  4. Which part of the onion root should you use for the mitosis slide?

    (1) The base (closest to the bulb)

    (2) The tip (fartherest away from the bulb)

    (3) The middle part of the root

    (4) The bulb

    (5) The young, leafy, green part

  5. Where does cleavage take place in the cell cycle?

    (1) interphase

    (2) prophase

    (3) metaphase

    (4) anaphase

    (5) telophase