Laboratory Study Guide,

Complex Single Cells: Protozoa

Type your email address in the space provided.

Type your full name in the space provided.

Read:

Complex Single Cells: Protozoa, pp 1-7.

Terms to Know in This Chapter:

prokaryotic [pro care ee OT ik]

eukaryotic [you care ee OT ik]

nucleus (sing.) NEW klee us]

nuclei (pl.) [NEW klee eye]

cytoplasm [SIGH toe plaz um]

protoplasm [PRO toe plaz um]

cell membrane

autotroph (sing.) [AW toe trof]

heterotroph (sing.) [HET er oh trof]

glucose [GLUE kose]

glycerol [GLIS er ol]

fatty acids

amino acid (sing.) [ah MEAN oh]

nucleotide (sing.) [NEW klee oh tide]

vitamin (sing.) [VIE tah men]

intracellular [in trah CELL you lar]

phagocytosis [fag oh sigh TOE siss]

pinocytosis [pee no sigh TOE siss]

vacuole (sing.) [VAK youl]

extracellular [ex trah CELL you lar]

hydrolysis [high DROL oh siss]

respiration [ress peh RAY shun]

micronucleus (sing.) [my crow NEW klee us]

micronuclei (pl.) [my crow NEW klee eye]

macronucleus (sing.) [mak row NEW klee us]

macronuclei (pl.) [mak row NEW klee eye]

oral groove (sing.)

taxis (sing.) [TAX iss]

taxes (pl.) [TAX ez]

cilium (sing.) [SILL ee um]

cilia (pl.) [SILL ee ah]

digestion [die GESS ghun]

yeast (sing.)

Congo red

covered [ah see toe OR seen]

methyl cellulose [METH ul] [CELL you loas]

lysosome (sing.) [LIE so zome]

hydrochloric acid

anal pore (sing.)

contractile vacuole (sing.) [con TRACK tul]

fission [FIZ un]

centimeter (sing.) [SIN teh meet ur]

millimeter (sing.) [MILL eh meet ur]

micron (sing.) [MY kron]

Genera You Need to Know

Paramecium [pear ah ME see um]

What You Need to Know:

You should be able to:

1. distinguish between prokaryotic and eukaryotic cells,
2. name the major parts of a eukaryotic cell,
3. distinguish between autotrophs and heterotrophs,
4. distinguish between intracellular and extracellular digestion,
5. distinguish between pinocytosis and phagocytosis,
6. make a wet mount of living Paramecium and study the movement of the organism,
7. demonstrate the ability to use proper staining and slowing techniques in lab,
8. define what is meant by taxis,
9. measure and approximate the size of a Paramecium as explained in the laboratory exercise,
10. explain the uses of acetoorcein, methyl cellulose and Congo red,
11. distinguish between a macro- and micronucleus and explain the functions of each,
12. explain what is happening in the food vacuoles and lysosomes of Paramecium and relate to extra- and intracellular digestion,
13. demonstrate the ability to observe the contractions of contractile vacuoles and explain what is happening in relation to various environments to which the Paramecium might be exposed, and
14. define fission.

Exercises: Fill in the Blank.

1. The use of inorganic carbon to form glucose via photosynthesis occurs in an organism called a(n) .
2. Digestion that occurs inside the cell is called digestion.
3. The is responsible for every day metabolism of Paramecium and the is involved in sexual reproduction.
4. Paramecium moves by using short, hair-like filaments called .
5. How many contractile vacuoles are found in Paramecium?
6. If an object appears to be 2.5 cm in your microscope and you are using 430 magnification, how many microns in size is the organism? .
7. Which stain is utilized to better see the macro- and micronucleus?
8. What happens to the Congo red-stained yeast cells as time goes by? (Text will scroll)
9. Will the contractile vacuole work faster or slower when Paramecium is placed in a 0.5% salt solution? .
10. Asexual reproduction in Paramecium is called .

Exercises: Multiple Choice. Select the Best Answer.

1. How many nuclei does Paramecium have?
   

   (1) one
   

   (2) two
   

   (3) three: two macro- and one micro-
   

   (4) three: one macro- and two micro-
   

   (5) it varies

2. Which of the following is a true statement?

   (1) Acetoorcein turns blue in the presence of an acid.
   

   (2) Paramecium has three contractile vacuoles.
   

   (3) Contractile vacuoles work faster in distilled water environments.
   

   (4) The macronucleus is involved in sexual reproduction.
   

   (5) More than one answer is correct.

3. Digestion of food inside the Paramecium is called
   

   (1) intracellular.
   

   (2) extracellular.
   

   (3) autotrophism.
   

   (4) saprotrophism.
   

   (5) None of the above.

4. Which dye is used to stain nuclei?
   

   (1) methyl green
   

   (2) Congo Red
   

   (3) acetoorcein
   

   (4) iodine
   

   (5) None of these.

5. What is a micron?
   

   (1) a millimeter.
   

   (2) 100 millimeters
   

   (3) 1000 millimeters
   

   (4) 1,000,000 millimeters
   

   (5) None of the above are correct.