Laboratory Study Guide,

The Cell Membrane

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Read:

The Cell Membrane, pp 1-5.

Terms to Know in This Chapter:

reproduction [re pro DUCT shun]

metabolism [meh TAB oh liz um]

mutation [mew TAY shun]

stimulus (sing.) [STIM you lus]

stimuli (pl.) [STIM you lye]

micron (sing.) [MY kron]

active transport

secretion [seh KREE shun]

excretion [ex KREE shun]

gradient [GRAY dee unt]

Brownian movement [BROW ee un]

molecular motion

carmine [CAR mine]

diffusion [deh FUSE on]

osmosis [os MOE siss]

selectively permeable [seh LEK tive lee] [PUR me ah bul]

osmotic pressure

dialysis [die AL ah siss]

sucrose [SUE krose]

glucose [GLUE kose]

iodine [EYE oh dine]

starch

Testape [TEST tape]

salt solution

hypotonic [high poe TAWN ik]

hypertonic [high per TAWN ik]

isotonic [eye so TAWN ik]

turgor [TUR gor]

turgid [TUR gid]

plasmolysis [plaz MOLL eh siss]

cytolysis [sigh TOLL oh siss]

hemolysis [he MOLL oh siss]

crenolysis [creh NOLL eh siss]

Ringer's solution

saline solution [SAY leen]

yeast (sing.)

Congo red [CON go]

Genera You Need to Know

Elodea [el oh DEE ah] or [ee LOAD ee ah]

People You Need to Know

Robert Brown

What You Need to Know:

You should be able to:

1. name some characteristics of living matter,
2. describe the cell membrane according to size, functions, and permeability,
3. define Brownian movement and be able to demonstrate it in lab,
4. explain why carmine dye particles randomly move in solution,
5. demonstrate osmotic pressure,
6. define diffusion and osmosis,
7. explain how particle size matters in diffusion and demonstrate the process in lab, and run various diagnostic tests to prove movement of particles,
8. demonstrate turgor and plasmolysis in cells,
9. define hypotonic, hypertonic, and isotonic and demonstate each in living cells,
10. demonstrate hemolysis and crenolysis in cells, and
11. explain the experiment utilizing yeast cells and membrane permeability.

Exercises: Fill in the Blank.

1. Characteristics of living organisms may include
   a.
   b.
   c. , and
   d. .
2. A membrane that allows the passage of some materials but blocks the passage of others is termed .
3. The apparent random motion of molecules is called movement.
4. The passage of particles from a region of higher concentration to a region of lower concentration is called while the diffusion of materials through a membrane is called .
5. In the experiment of osmotic pressure, what was the direction of flow of water? (Text will scroll.) .
6. In the experiment where you placed starch and glucose solution in a dialysis bag and then placed that in a beaker of water containing iodine, what happened to the sugar in the bag? (Text will scroll.)
7. Which particle in the previous question was the smallest, iodine, glucose, starch? .
8. A potato placed in a hypertonic solution should experience .
9. A 5% salt solution placed on a potato strip would be this type of solution. (isotonic, hypertonic, hypotonic)
10. Place Elodea in a distilled water solution and it will experience (turgor, plasmolysis)
11. Place red blood cells in a hypertonic environment and they will undergo while a red blood cell placed in a hypotonic environment would undergo .
12. Why did the yeast that was boiled with Congo Red turn red inside the cell? (Text will scroll.)

Exercises: Multiple Choice. Select the Best Answer.

1. What happens when you place Elodea in a hypotonic environment?
   

   (1) Water will diffuse into the cell.
   

   (2) The cell will undergo turgor.
   

   (3) The cell will undergo plasmolysis.
   

   (4) Water will leave the cell.
   

   (5) Both 1 and 2 are correct.

2. The apparent random motion of atoms, ions, and molecules is called

   (1) diffusion.
   

   (2) osmosis.
   

   (3) Brownian movement.
   

   (4) plasmolysis.
   

   (5) osmotic pressure.

3. Which of the following is the largest particle?
   

   (1) iodine
   

   (2) glucose
   

   (3) sucrose
   

   (4) starch
   

   (5) fructose

4. Which of the following is the correct sequence of smallest to largest particle?
   

   (1) iodine, glucose, sucrose, starch
   

   (2) iodine, sucrose, glucose, starch
   

   (3) glucose, sucrose, iodine, starch
   

   (4) starch, sucrose, glucose, iodine
   

   (5) starch, glucose, sucrose, iodine

5. The passage of particles from a region of higher concentration to a region of lower concentration is called
   

   (1) Brownian movement.
   

   (2) osmosis.
   

   (3) diffusion.
   

   (4) plasmolysis.
   

   (5) hydrolysis.